Genotoxicity testing of esterified propoxylated glycerol (EPG)

Genotoxicity Testing: a Review

Genotoxicity is a major limitation of various compounds used in clinical practices which range from gene mutations, chromosomal aberrations to other DNA effects. For the same reason, it must be assessed before bringing into the market. Moreover, various regulatory agencies have made it mandatory to submit the genotoxicity data while filing a New Drug Application (NDA). This in turn has given fu...

High-Throughput Genotoxicity Testing

The registration of all pharmaceuticals requires a comprehensive safety assessment of their potential to cause direct or indirect genotoxic damage. This article reviews current methods employed for regulatory genotoxicity testing and new techniques developed for early high-throughput screening. A novel in vitro mammalian assay is described which detects all common mechanistic classes of genotox...

Concentrations of glycerol and non-esterified fatty acids in plasma during fat mobilization.

The presence of fat-mobilizing activity in the pituitary gland was first demonstrated by Best & Campbell (1936). More recently a series of studies have been carried out on a lipid-mobilizing factor which has been extensively purified from the anterior pituitary gland (Rudman & Seidman, 1958; Rudman, Seidman & Reid, 1960; Di Girolamo, Rudman, Reid & Seidman, 1961; Rudman, Seidman, Brown & Hirsch...

Appropriate In Vitro Methods for Genotoxicity Testing of Silver Nanoparticles

OBJECTIVES We investigated the genotoxic effects of 40-59 nm silver nanoparticles (Ag-NPs) by bacterial reverse mutation assay (Ames test), in vitro comet assay and micronucleus (MN) assay. In particular, we directly compared the effect of cytochalasin B (cytoB) and rat liver homogenate (S9 mix) in the formation of MN by Ag-NPs. METHODS Before testing, we confirmed that Ag-NPs were completely...

New Trends in Genotoxicity Testing of Herbal Medicinal Plants